Recombinant
Recombinant protein kits are convenient tools designed to facilitate the expression and purification of recombinant proteins in a laboratory setting. These kits typically include all the necessary components and reagents required for protein expression, purification, and often quantification.
Here are some common components you might find in a recombinant protein kit:
Expression vector:
Host cells:
Depending on the kit, it may include competent cells for bacterial transformation, yeast cells, or mammalian cell lines suitable for transfection.
Inducers:
Some kits include inducers (such as IPTG for bacterial systems) to initiate protein expression once the host cells have been transformed or transfected with the expression vector.
Purification reagents:
Kits typically include reagents and materials for protein purification, such as affinity resins, chromatography columns, and buffers optimized for protein purification.
Detection and quantification reagents:
Many kits also include reagents for detecting and quantifying the expressed protein, such as antibodies specific to the protein of interest or colorimetric/fluorescent substrates for enzyme assays.
Instructions and protocols:
Detailed protocols and instructions for using the kit are provided to guide users through the expression and purification process.
AffiREC® offers a comprehensive portfolio of recombinant proteins spanning a wide spectrum of targets and functions. From antibodies to enzymes, our extensive collection empowers you to explore the intricate web of biological mechanisms.