PCR Products
In the realm of molecular biology and genetic analysis, a suite of specialized reagents has revolutionized the way researchers approach nucleic acid quantification and amplification. From the sensitive detection of RNA targets to the precise amplification of DNA sequences, these advanced solutions streamline complex workflows and deliver reliable results.
At the forefront is the 4X CAPITAL TM 1-Step qRT-PCR Probe Master Mix, a comprehensive reagent blend designed for quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays. This all-in-one mix simplifies the process by providing researchers with everything needed for reverse transcription and PCR amplification in a single tube. With its inclusion of reverse transcriptase, DNA polymerase, probes, primers, and nucleotides, alongside buffers and stabilizers, accurate quantification of RNA targets becomes effortless.
Complementing this is the SCRIPT Direct RT-qPCR ProbesMaster UNG, offering a similar convenience with added benefits of Uracil-N-Glycosylase (UNG). This enzyme safeguards against contamination by cleaving uracil bases from DNA, ensuring the fidelity of results in RT-qPCR assays.
For conventional PCR applications, the 2X YourTaq TM Direct-Load Hot-Start PCR Master Mix stands out. Engineered with a hot-start enzyme to minimize non-specific amplification and primer-dimer formation, this mix simplifies PCR workflows while guaranteeing high specificity and efficiency. Its 'Direct-Load' feature enables direct loading of PCR products onto agarose gels post-amplification, saving time and minimizing handling steps.
Rounding out the selection is the 4X CAPITAL TM qPCR Green Master Mix LROX, tailored for quantitative PCR (qPCR) assays. Equipped with DNA polymerase, dNTPs, buffers, magnesium, and both green fluorescent and ROX dyes, this mix enables real-time detection and normalization of fluorescence signals, ensuring precise quantification of DNA targets.
Together, these advanced reagent mixes represent a paradigm shift in nucleic acid analysis, empowering researchers with the tools needed to unlock the secrets of the genome and transcriptome with unprecedented speed, accuracy, and convenience.
4X CAPITAL TM 1-Step qRT-PCR Probe Master Mix:
This product is a ready-to-use reagent mix specifically designed for quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays that incorporate a probe-based detection method. It simplifies the workflow by providing a pre-mixed solution containing all necessary components for reverse transcription and PCR amplification in a single tube. The mix includes reverse transcriptase, DNA polymerase, probes, primers, nucleotides, buffer, and stabilizers/enhancers. It enables researchers to efficiently and accurately quantify RNA targets by amplifying complementary DNA (cDNA) synthesized from RNA templates and detecting the amplified products in real-time using fluorescent probes.
SCRIPT Direct RT-qPCR Probes Master UNG:
This product is another ready-to-use reagent mix tailored for quantitative reverse transcription polymerase chain reaction (RT-qPCR) assays with probe-based detection. It includes reverse transcriptase, DNA polymerase with Uracil-N-Glycosylase (UNG), probes, primers, nucleotides, buffer, and stabilizers/enhancers. The presence of UNG helps prevent carryover contamination by cleaving uracil bases from DNA. This master mix streamlines the RT-qPCR workflow by providing all necessary components for reverse transcription and PCR amplification in a single tube, facilitating accurate and sensitive quantification of RNA targets.
2X YourTaq TM Direct-Load Hot-Start PCR Master Mix:
This product is a ready-to-use reagent mix designed for standard PCR assays. It contains YourTaq DNA polymerase, a hot-start enzyme that minimizes non-specific amplification and primer-dimer formation by inhibiting the enzyme's activity until the reaction reaches the optimal annealing temperature. The mix also includes dNTPs, buffer, magnesium, stabilizers/enhancers, and other components. The "Direct-Load" feature allows users to load PCR products directly onto agarose gels for analysis after amplification without additional steps. This master mix offers convenience, efficiency, and high specificity for a wide range of PCR applications.
4X CAPITAL TM qPCR Green Master Mix LROX:
This product is a specialized reagent mix formulated for quantitative PCR (qPCR) assays. It contains DNA polymerase, dNTPs, buffer, magnesium, ROX dye (Low Concentration ROX), green fluorescent dye, and stabilizers/enhancers. The green fluorescent dye enables real-time detection of PCR products, while the ROX dye serves as a passive reference dye for normalization of fluorescence signals. This master mix simplifies the qPCR workflow by providing all necessary components for PCR amplification in a single tube, facilitating accurate and reproducible quantification of DNA targets.
